부산대학교 도서관

Methods and Results: Cardiac myoblast H9c2 cells were treated with AGEs (100 or 200 μg/mL). AGEs increased the protein levels of p53 and γ-H2AX as well as the gene expression levels of p21 and p53. Next, we examined a mouse model of type 2 diabetes. C57BL6/N mice (8-week-old, male) were fed a high-fat diet (HFD) and L-NAME (1 g/L) for 4 weeks, followed by daily injections of streptozotocin (STZ) (50 mg/kg/day) for 5 days and sacrificed after another 4 weeks. Picrosirius red staining showed that cardiac fibrosis was increased in L-NAME+HFD+STZ (LHS) mice. LHS mice showed increased gene expression levels of inflammatory cytokines, fibrosis-related genes, and senescence markers. Acetylation and crotonylation levels of histone H3K9 were enhanced in LHS mice.