e-Article
A genome catalog of the early-life human skin microbiome
Document Type
Academic Journal
Author
Shen, Zeyang; Robert, Lukian; Stolpman, Milan; Che, You; Allen, Katrina J.; Saffery, Richard; Walsh, Audrey; Young, Angela; Eckert, Jana; Deming, Clay; Chen, Qiong; Conlan, Sean; Laky, Karen; Li, Jenny Min; Chatman, Lindsay; Kashaf, Sara Saheb; Barnabas, Beatrice B.; Black, Sean; Bouffard, Gerard G.; Brooks, Shelise Y.; Crawford, Juyun; Marfani, Holly; Dekhtyar, Lyudmila; Han, Joel; Ho, Shi-Ling; Legaspi, Richelle; Maduro, Quino L.; Masiello, Catherine A.; McDowell, Jennifer C.; Montemayor, Casandra; Mullikin, James C.; Park, Morgan; Riebow, Nancy L.; Schandler, Karen; Schmidt, Brian; Sison, Christina; Stantripop, Sirintorn; Thomas, James W.; Thomas, Pamela J.; Vemulapalli, Meghana; Young, Alice C.
Source
Genome Biology (Online Edition). November 10, 2023, Vol. 24 Issue 1
Subject
Language
English
Abstract
Author(s): Zeyang Shen[sup.1], Lukian Robert[sup.1], Milan Stolpman[sup.2], You Che[sup.2], Katrina J. Allen[sup.3,4,5], Richard Saffery[sup.4,5], Audrey Walsh[sup.3,5], Angela Young[sup.3,5], Jana Eckert[sup.3,5], Clay Deming[sup.1], Qiong Chen[sup.1], Sean Conlan[sup.1], Karen Laky[sup.6], Jenny Min [...]
Background Metagenome-assembled genomes have greatly expanded the reference genomes for skin microbiome. However, the current reference genomes are largely based on samples from adults in North America and lack representation from infants and individuals from other continents. Results Here we use deep shotgun metagenomic sequencing to profile the skin microbiota of 215 infants at age 2-3 months and 12 months who are part of the VITALITY trial in Australia as well as 67 maternally matched samples. Based on the infant samples, we present the Early-Life Skin Genomes (ELSG) catalog, comprising 9483 prokaryotic genomes from 1056 species, 206 fungal genomes from 13 species, and 39 eukaryotic viral sequences. This genome catalog substantially expands the diversity of species previously known to comprise human skin microbiome and improves the classification rate of sequenced data by 21%. The protein catalog derived from these genomes provides insights into the functional elements such as defense mechanisms that distinguish early-life skin microbiome. We also find evidence for microbial sharing at the community, bacterial species, and strain levels between mothers and infants. Conclusions Overall, the ELSG catalog uncovers the skin microbiome of a previously underrepresented age group and population and provides a comprehensive view of human skin microbiome diversity, function, and development in early life.
Background Metagenome-assembled genomes have greatly expanded the reference genomes for skin microbiome. However, the current reference genomes are largely based on samples from adults in North America and lack representation from infants and individuals from other continents. Results Here we use deep shotgun metagenomic sequencing to profile the skin microbiota of 215 infants at age 2-3 months and 12 months who are part of the VITALITY trial in Australia as well as 67 maternally matched samples. Based on the infant samples, we present the Early-Life Skin Genomes (ELSG) catalog, comprising 9483 prokaryotic genomes from 1056 species, 206 fungal genomes from 13 species, and 39 eukaryotic viral sequences. This genome catalog substantially expands the diversity of species previously known to comprise human skin microbiome and improves the classification rate of sequenced data by 21%. The protein catalog derived from these genomes provides insights into the functional elements such as defense mechanisms that distinguish early-life skin microbiome. We also find evidence for microbial sharing at the community, bacterial species, and strain levels between mothers and infants. Conclusions Overall, the ELSG catalog uncovers the skin microbiome of a previously underrepresented age group and population and provides a comprehensive view of human skin microbiome diversity, function, and development in early life.