e-Article
Genetic Variation of the Vitamin D Binding Protein Affects Vitamin D Status and Response to Supplementation in Infants
CLINICAL RESEARCH ARTICLE
CLINICAL RESEARCH ARTICLE
Document Type
Clinical report
Author
Source
Journal of Clinical Endocrinology & Metabolism. November 2019, Vol. 104 Issue 11, p5483, 16 p.
Subject
Language
English
ISSN
0021-972X
Abstract
Vitamin D insufficiency is common worldwide (1). Many countries have implemented recommendations of vitamin D supplementation and vitamin D fortification of food products (2-4). Supplementation is particularly important during infancy [...]
Context: Single nucleotide polymorphisms (SNPs) of the vitamin D binding protein encoding the GC (group component) gene affect 25-hydroxyvitamin D (25OHD) concentrations, but their influence on vitamin D status and response to vitamin D supplementation in infants is unknown. Objective: To study GC genotype--related differences in 25OHD concentrations and the response to supplementation during a vitamin D intervention study in infants. Design: In this randomized controlled trial, healthy term infants received vitamin [D.sub.3] (10 or 30 [micro]g/d) from 2 weeks to 24 months of age. GC SNPs rs2282679, rs4588, rs7041, and rs1155563 were genotyped. rs4588/7041 diplotype and haplotypes of rs2282679, rs4588, and rs7041 ([Haplo.sub.3SNP]) and of all four SNPs ([Haplo.sub.4SNP]) were determined. Main Outcome Measures: 25OHD measured in cord blood at birth and at 12 and 24 months during intervention. Results: A total of 913 infants were included. Minor allele homozygosity of all studied GC SNPs, their combined haplotypes, and rs4588/rs7041 diplotype 2/2 were associated with lower 25OHD concentrations at all time points in one or both intervention groups [analysis of covariance (ANCOVA) P < 0.043], with the exception of rs7041, which did not affect 25OHD at birth. In the highdose supplementation group receiving 30 [micro]g/d vitamin [D.sub.3], but not in those receiving 10 [micro]g/d, genotype of rs2282679, rs4588, and rs7041; diplotype; and [Haplo.sub.3SNP] significantly affected intervention response (repeated measurement ANCOVA [P.sub.interaction] < 0.019). Minor allele homozygotes had lower 25OHD concentrations and smaller increases in 25OHD throughout the intervention. Conclusions: In infants, vitamin D binding protein genotype affects 25OHD concentration and efficiency of high-dose vitamin [D.sub.3] supplementation. (J Clin Endocrinol Metab 104: 5483-5498, 2019)
Context: Single nucleotide polymorphisms (SNPs) of the vitamin D binding protein encoding the GC (group component) gene affect 25-hydroxyvitamin D (25OHD) concentrations, but their influence on vitamin D status and response to vitamin D supplementation in infants is unknown. Objective: To study GC genotype--related differences in 25OHD concentrations and the response to supplementation during a vitamin D intervention study in infants. Design: In this randomized controlled trial, healthy term infants received vitamin [D.sub.3] (10 or 30 [micro]g/d) from 2 weeks to 24 months of age. GC SNPs rs2282679, rs4588, rs7041, and rs1155563 were genotyped. rs4588/7041 diplotype and haplotypes of rs2282679, rs4588, and rs7041 ([Haplo.sub.3SNP]) and of all four SNPs ([Haplo.sub.4SNP]) were determined. Main Outcome Measures: 25OHD measured in cord blood at birth and at 12 and 24 months during intervention. Results: A total of 913 infants were included. Minor allele homozygosity of all studied GC SNPs, their combined haplotypes, and rs4588/rs7041 diplotype 2/2 were associated with lower 25OHD concentrations at all time points in one or both intervention groups [analysis of covariance (ANCOVA) P < 0.043], with the exception of rs7041, which did not affect 25OHD at birth. In the highdose supplementation group receiving 30 [micro]g/d vitamin [D.sub.3], but not in those receiving 10 [micro]g/d, genotype of rs2282679, rs4588, and rs7041; diplotype; and [Haplo.sub.3SNP] significantly affected intervention response (repeated measurement ANCOVA [P.sub.interaction] < 0.019). Minor allele homozygotes had lower 25OHD concentrations and smaller increases in 25OHD throughout the intervention. Conclusions: In infants, vitamin D binding protein genotype affects 25OHD concentration and efficiency of high-dose vitamin [D.sub.3] supplementation. (J Clin Endocrinol Metab 104: 5483-5498, 2019)