부산대학교 도서관

A protein-G sepharose affinity chromatography was validated to quantify antibodies from cell culture supernatants. The calibration curve linear range was 30--480 ug immunoglobulin (IgG). Between-run study demonstrated a coefficient of variation 5.73--9.02% in 20 purification cycles and sample dilution with protein-free medium (>3 mL) showed a marked IgG amount over estimation. Sensitivity was 30 ug, and bovine serum albumin addition to columns did not affect IgG recovery (a90%). CB.Ifn-2.4 antibody quantification in MiniPERM.sup.(r) bioreactor supernatant reached 1.5 mg mL.sup.-1, coinciding with concentration measured by ELISA and thus demonstrating the protein-G sepharose column accuracy used to quantify IgG under these experimental conditions.