e-Article
Dynamic establishment and maintenance of the human intestinal B cell population and repertoire following transplantation in a pediatric-dominated cohort
Document Type
article
Author
Jianing Fu; Thomas Hsiao; Elizabeth Waffarn; Wenzhao Meng; Katherine D. Long; Kristjana Frangaj; Rebecca Jones; Alaka Gorur; Areen Shtewe; Muyang Li; Constanza Bay Muntnich; Kortney Rogers; Wenyu Jiao; Monica Velasco; Rei Matsumoto; Masaru Kubota; Steven Wells; Nichole Danzl; Shilpa Ravella; Alina Iuga; Elena-Rodica Vasilescu; Adam Griesemer; Joshua Weiner; Donna L. Farber; Eline T. Luning Prak; Mercedes Martinez; Tomoaki Kato; Uri Hershberg; Megan Sykes
Source
Frontiers in Immunology, Vol 15 (2024)
Subject
Language
English
ISSN
1664-3224
91097401
91097401
Abstract
IntroductionIt is unknown how intestinal B cell populations and B cell receptor (BCR) repertoires are established and maintained over time in humans. Following intestinal transplantation (ITx), surveillance ileal mucosal biopsies provide a unique opportunity to map the dynamic establishment of recipient gut lymphocyte populations in immunosuppressed conditions.MethodsUsing polychromatic flow cytometry that includes HLA allele group-specific antibodies distinguishing donor from recipient cells along with high throughput BCR sequencing, we tracked the establishment of recipient B cell populations and BCR repertoire in the allograft mucosa of ITx recipients.ResultsWe confirm the early presence of naïve donor B cells in the circulation (donor age range: 1-14 years, median: 3 years) and, for the first time, document the establishment of recipient B cell populations, including B resident memory cells, in the intestinal allograft mucosa (recipient age range at the time of transplant: 1-44 years, median: 3 years). Recipient B cell repopulation of the allograft was most rapid in infant (