부산대학교 도서관

The polyphagous insect, Frankliniella occidentalis (Pergande) (Fo), causes serious damage to economic plants in the world, whereas it is not found on the list of quarantine pests in Taiwan. However, the pest is occasionally found in imported agriculture products. In this study, molecular characters were used to identify the nymph stage of this quarantine pest; the onion thrips, Thrips tabaci Lindeman, found mostly in imported agriculture products was used as a reference sample. Molecular markers including both 28S rDNA and 16S rDNA sequences were analyzed. For 28S rDNA, 22 individuals of these two species were used, and intraspecific sequence variation were not found; interspecific variation was 12.3% in 689 bp. For 16S rDNA, 18 individuals of these two species were used, and intraspecific sequence variations were nearly zero, while interspecific variation was 17.5% between these two species. Specific primers designed from 28S rDNA and restriction enzyme from 16S rDNA were respectively applied for multiplex- PCR and PCR-RFLP to identify F. occidntalis. Results showed that application of the two molecular markers was useful in identifying this quarantine pest.