e-Article
A sensitive assay for active link protein from cartilage
Document Type
Article
Author
Source
Biochemical Journal; December 1985, Vol. 232 Issue: 2 p613-616, 4p
Subject
Language
ISSN
02646021; 14708728
Abstract
A new assay for the activity of cartilage link protein is described. The method is based on the finding [Plaas, Sandy & Muir (1983) Biochem. J. 214, 855-864] that addition of link protein to [35S]sulphate-labelled proteoglycan aggregates from rabbit chondrocyte cultures resulted in the formation of link-stabilized aggregates. The percentage aggregate was found to be related linearly to the amount of purified bovine link protein added in the 20-120 ng range. The assay was used to monitor loss of link-protein activity during heat denaturation and to measure binding of link protein by purified proteoglycan monomer.