e-Article
Bisphenol S Impairs Oestradiol Secretion during In Vitro Basal Folliculogenesis in a Mono-Ovulatory Species Model.
Document Type
Academic Journal
Author
Vignault C; CNRS, IFCE, INRAE, Université de Tours, PRC, 37380 Nouzilly, France.; Service de Médecine et Biologie de la Reproduction, CHRU de Tours, 37000 Tours, France.; Cadoret V; CNRS, IFCE, INRAE, Université de Tours, PRC, 37380 Nouzilly, France.; Service de Médecine et Biologie de la Reproduction, CHRU de Tours, 37000 Tours, France.; Jarrier-Gaillard P; CNRS, IFCE, INRAE, Université de Tours, PRC, 37380 Nouzilly, France.; Papillier P; CNRS, IFCE, INRAE, Université de Tours, PRC, 37380 Nouzilly, France.; Téteau O; CNRS, IFCE, INRAE, Université de Tours, PRC, 37380 Nouzilly, France.; Desmarchais A; CNRS, IFCE, INRAE, Université de Tours, PRC, 37380 Nouzilly, France.; Uzbekova S; CNRS, IFCE, INRAE, Université de Tours, PRC, 37380 Nouzilly, France.; Binet A; CNRS, IFCE, INRAE, Université de Tours, PRC, 37380 Nouzilly, France.; Service de Chirurgie Pédiatrique Viscérale, Urologique, Plastique et Brûlés, CHRU de Tours, 37000 Tours, France.; Guérif F; CNRS, IFCE, INRAE, Université de Tours, PRC, 37380 Nouzilly, France.; Service de Médecine et Biologie de la Reproduction, CHRU de Tours, 37000 Tours, France.; Elis S; CNRS, IFCE, INRAE, Université de Tours, PRC, 37380 Nouzilly, France.; Maillard V; CNRS, IFCE, INRAE, Université de Tours, PRC, 37380 Nouzilly, France.
Source
Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101639637 Publication Model: Electronic Cited Medium: Internet ISSN: 2305-6304 (Electronic) Linking ISSN: 23056304 NLM ISO Abbreviation: Toxics Subsets: PubMed not MEDLINE
Subject
Language
English
Abstract
Bisphenol S (BPS) affects terminal folliculogenesis by impairing steroidogenesis in granulosa cells from different species. Nevertheless, limited data are available on its effects during basal folliculogenesis. In this study, we evaluate in vitro the effects of a long-term BPS exposure on a model of basal follicular development in a mono-ovulatory species. We cultured ovine preantral follicles (180−240 μm, n = 168) with BPS (0.1 μM (possible human exposure dose) or 10 μM (high dose)) and monitored antrum appearance and follicular survival and growth for 15 days. We measured hormonal secretions (oestradiol (at day 13 [D13]), progesterone and anti-Müllerian hormone [D15]) and expression of key follicular development and redox status genes (D15) in medium and whole follicles, respectively. BPS (0.1 µM) decreased oestradiol secretion compared with the control (−48.8%, p < 0.001), without significantly impairing antrum appearance, follicular survival and growth, anti-Müllerian hormone and progesterone secretion and target gene expression. Thus, BPS could also impair oestradiol secretion during basal folliculogenesis as it is the case during terminal folliculogenesis. It questions the use of BPS as a safe BPA substitute in the human environment. More studies are required to elucidate mechanisms of action of BPS and its effects throughout basal follicular development.