e-Article
Preclinical Study of DCD and Normothermic Perfusion for Visceral Transplantation.
Document Type
Article
Author
Serradilla, Javier; Andrés Moreno, Ane Miren; Talayero, Paloma; Burgos, Paula; Machuca, Mariana; Ortega, Onys Camps; Vallejo, María Teresa; Rubio Bolívar, Francisco Javier; Bueno, Alba; Sánchez, Alba; Zambrano, Cristina; De la Torre Ramos, Carlos Andrés; Rodríguez, Olaia; Largo, Carlota; Serrano, Pilar; Prieto Bozano, Gerardo; Ramos, Esther; López Santamaría, Manuel; Stringa, Pablo; Hernández, Francisco
Source
Subject
*SMALL intestine
*ISOLATION perfusion
*PERFUSION
*GRAFT rejection
*REPERFUSION injury
*SHORT bowel syndrome
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Language
ISSN
0934-0874
Abstract
Considering recent clinical and experimental evidence, expectations for using DCDderived intestines have increased considerably. However, more knowledge about DCD procedure and long-term results after intestinal transplantation (ITx) is needed. We aimed to describe in detail a DCD procedure for ITx using normothermic regional perfusion (NRP) in a preclinical model. Small bowel was obtained from pigs donors after 1 h of NRP and transplanted to the recipients. Graft Intestinal samples were obtained during the procedure and after transplantation. Ischemia-reperfusion injury (Park-Chiu score), graft rejection and transplanted intestines absorptive function were evaluated. Seven of 8 DCD procedures with NRP and ITx were successful (87.5%), with a good graft reperfusion and an excellent recovery of the recipient. The architecture of grafts was well conserved during NRP. After an initial damage of Park-chiu score of 4, all grafts recovered from ischemia-reperfusion, with no or very subtle alterations 2 days after ITx. Most recipients (71.5%) did not show signs of rejection. Only two cases demonstrated histologic signs of mild rejection 7 days after ITx. Interestingly intestinal grafts showed good absorptive capacity. The study's results support the viability of intestinal grafts from DCD using NRP, contributing more evidence for the use of DCD for ITx. [ABSTRACT FROM AUTHOR]