e-Article
Performance of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Assays in Different Stages of Infection: Comparison of Commercial Enzyme-Linked Immunosorbent Assays and Rapid Tests.
Document Type
Journal Article
Author
Traugott, Marianna; Aberle, Stephan Walter; Aberle, Judith Helene; Griebler, Hannah; Karolyi, Mario; Pawelka, Erich; Puchhammer-Stöckl, Elisabeth; Zoufaly, Alexander; Weseslindtner, Lukas; Traugott, M; Aberle, S W; Aberle, J H; Griebler, H; Karolyi, M; Pawelka, E; Puchhammer-Stöckl, E; Zoufaly, A; Weseslindtner, L
Source
Subject
*ENZYME-linked immunosorbent assay
*COVID-19
*IMMUNOGLOBULINS
*
*
Language
ISSN
0022-1899
Abstract
We comparatively assessed sensitivities and specificities of 4 commercial enzyme-linked immunosorbent assays (ELISAs) and 2 rapid tests in 77 patients with polymerase chain reaction-confirmed severe acute respiratory syndrome coronavirus 2 infection, grouped by interval since symptom onset. Although test sensitivities were low (<40%) within the first 5 days after disease onset, immunoglobulin (Ig) M, IgA, and total antibody ELISAs increased in sensitivity to >80% between days 6 and 10 after symptom onset. The evaluated tests (including IgG and rapid tests) provided positive results in all patients at or after the 11th day after onset of disease. The specificities of the ELISAs were 83% (IgA), 98% (IgG), and 97% (IgM and total antibody). [ABSTRACT FROM AUTHOR]