e-Article
Experimental test of the geometric model of image formation in bright‐field microscopy.
Document Type
Article
Author
Source
Subject
*MICROSCOPY
*FOCAL planes
*GEOMETRIC modeling
*GEOMETRIC approach
*RAY tracing
*NEAR-field microscopy
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Language
ISSN
0022-2720
Abstract
Lay Description: It has become customary to analyse microscopic images in terms of diffraction theory. However, when one is not interested in resolving fine details of an image, a much simpler and more intuitive geometric analysis based on ray tracing can be adequate. We applied geometric approach to analysis of bright‐field images of a small glass cylinder at different positions of the objective. Such an object would be very difficult to analyse using diffraction theory because of its high refractive index and steep boundaries. However, ray tracing produced a good match between theory and experiment. It can become a promising approach in bright‐field applications, such as quantitative phase imaging. [ABSTRACT FROM AUTHOR]