학술논문
Axolotl Transgenesis via Injection of I-SceI Meganuclease or Tol2 Transposon System
Document Type
Chapter
Source
Salamanders: Methods and Protocols. 10/23/2022. 2562:321-333
Subject
Language
English
ISSN
1064-3745
1940-6029
1940-6029
Abstract
The axolotl Axolotls ( Ambystoma mexicanum Ambystoma mexicanum (A. mexicanum) ) has been widely used as an animal model for studying development and regeneration. In recent decades, the use of genetic engineering to alter gene expression has advanced our knowledge on the fundamental molecular and cellular mechanisms, pointing us to potential therapeutic targets. We present a detailed, step-by-step protocol for axolotl Axolotls transgenesis using either I-SceI meganuclease Transgenesis meganuclease or the mini Tol2 Transgenesis Tol2 transposon system, by injection of purified DNA into one-cell stage eggs. We add useful tips on the site of injection and the viability of the eggs.