학술논문
Improving the Accuracy of Fitted Atomic Models in Cryo-EM Density Maps of Protein Assemblies Using Evolutionary Information from Aligned Homologous Proteins
Document Type
Chapter
Author
Carugo, Oliviero, Editor; Eisenhaber, Frank, Editor; Rakesh, Ramachandran; Srinivasan, Narayanaswamy; Walker, John M., Series editor
Source
Data Mining Techniques for the Life SciencesData Mining Techniques for the Life Sciences. 04/27/2016. 1415:193-209
Subject
Language
English
ISSN
1940-6029
1064-3745
1064-3745
Abstract
Cryo-Electron Microscopy (cryo-EM) has become an important technique to obtain structural insights into large macromolecular assemblies. However the resolution of the density maps do not allow for its interpretation at atomic level. Hence they are combined with high resolution structures along with information from other experimental or bioinformatics techniques to obtain pseudo-atomic models. Here, we describe the use of evolutionary conservation of residues as obtained from protein structures and alignments of homologous proteins to detect errors in the fitting of atomic structures as well as improve accuracy of the protein–protein interfacial regions in the cryo-EM density maps.