학술논문
Detection of Intracellular Cytokines
Document Type
Chapter
Author
Source
Transplantation Immunology: Methods and Protocols. 05/22/2013. 1034:353-358
Subject
Language
English
ISSN
1064-3745
1940-6029
1940-6029
Abstract
The intracellular cytokine method allows for a multiparametric readout by flow cytometry with precise phenotyping of the responding T cells. The intracellular cytokine staining of cells that have been fixed and permeabilized following a short-term activation and staining with antibodies to cell surface markers allows for identification of the cellular origin of the cytokine accumulated product.