학술논문

Detection of Intracellular Cytokines
Document Type
Chapter
Source
Transplantation Immunology: Methods and Protocols. 05/22/2013. 1034:353-358
Subject
Flow cytometry
Intracellular
Cytokines
Cell surface markers
Protein transport inhibitor
Cell permeabilization
Language
English
ISSN
1064-3745
1940-6029
Abstract
The intracellular cytokine method allows for a multiparametric readout by flow cytometry with precise phenotyping of the responding T cells. The intracellular cytokine staining of cells that have been fixed and permeabilized following a short-term activation and staining with antibodies to cell surface markers allows for identification of the cellular origin of the cytokine accumulated product.