학술논문
Isolating Urinary Extracellular Vesicles as Biomarkers for Diabetic Disease
Document Type
Chapter
Author
Gnudi, Luigi Prof., Editor; Long, David A. Dr., Editor; Barreiro, Karina; Huber, Tobias B.; Holthofer, Harry; Walker, John M., Series Editor
Source
Diabetic Nephropathy : Methods and ProtocolsDiabetic Nephropathy. 11/08/2019. 2067:175-188
Subject
Language
English
ISSN
1940-6029
1064-3745
1064-3745
Abstract
Extracellular vesicles are lipid bilayer enclosed structures secreted by all cell types. Their cargo includes proteins, lipids, RNAs, and DNA, which reflect the physiological state of their cells of origin. Recently, urinary extracellular vesicles have emerged as a valuable source of biomarkers for kidney and systemic disease.
Unfortunately, all existing methods for extracellular vesicle isolation from urine are time consuming and/or expensive. Thus, they are not adaptable to large-scale studies and unsuitable for clinical use without special equipment in the laboratory. Recently, our group has devised a set of new, quick, simple, and inexpensive techniques, based on hydrostatic filtration dialysis (HFD) of urine extremely suitable for diagnostic purposes. This novel approach represents a great potential for new diagnostics and understanding disease biology in general and brings the biomarker detection to the scope of all laboratories.
Unfortunately, all existing methods for extracellular vesicle isolation from urine are time consuming and/or expensive. Thus, they are not adaptable to large-scale studies and unsuitable for clinical use without special equipment in the laboratory. Recently, our group has devised a set of new, quick, simple, and inexpensive techniques, based on hydrostatic filtration dialysis (HFD) of urine extremely suitable for diagnostic purposes. This novel approach represents a great potential for new diagnostics and understanding disease biology in general and brings the biomarker detection to the scope of all laboratories.