부산대학교 도서관

Rab GTPase is critical for autophagy processes and is implicated in insect immunity against viruses. In this study, we aimed to investigate the role of FoRabsin the autophagic regulation of antiviral defense against tomato spotted wilt orthotospovirus (TSWV) in Frankliniella occidentalis. Transcriptome analysis revealed the downregulation of FoRabsin viruliferous nymph and adults of F. occidentalisin response to TSWV infection. Manipulation of autophagy levels with 3-MA and Rapa treatments resulted in a 5- to 15-fold increase and a 38–64% decrease in viral titers, respectively. Additionally, interference with FoRab10in nymphs and FoRab29in adults led to a 20–90% downregulation of autophagy-related genes, a decrease in ATG8-II (an autophagy marker protein), and an increase in the TSWV titers by 1.5- to 2.5-fold and 1.3- to 2.0-fold, respectively. In addition, the leaf disk and the living plant methods revealed increased transmission rates of 20.8–41.6 and 68.3–88.3%, respectively. In conclusion, FoRab10 and FoRab29 play a role in the autophagic regulation of the antiviral defense in F. occidentalisnymphs and adults against TSWV, respectively. These findings offer insights into the intricate immune mechanisms functional in F. occidentalisagainst TSWV, suggesting potential targeted strategies for F. occidentalisand TSWV management.