부산대학교 도서관

Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder, caused by both genetic and environmental variables. The rs9939609 variant with a T > A mutation of the Fat mass obesity (FTO) gene has been known to have a strong association with T2DM and extensively investigated for its role in T2DM in different populations. In this study we conducted a case–control study based on the Bangladeshi population and a combined updated meta-analysis using the data of previous studies from different populations and our study to investigate the association of rs9939609 with T2DM. A total of 156 diabetic patients and 144 healthy non-diabetic controls were recruited. ARMS (Amplification Refractory Mutation System) PCR method was used for genotyping and sequencing was done to validate the PCR results. Our study found a significant association (p < 0.05) of rs9939609 with T2DM in co-dominant AA vs. TT (OR = 2.64) dominant TA + AA vs. TT (OR = 2.12) and additive 2(AA) + TA(OR = 2.224) genetic models. From the meta-analysis, we found significant association of rs9939609 with T2DM in the overall population for all genetic models. However, subgroup analysis showed negligible association in the Caucasian population unlike the Asian population. This is the first case–control study conducted on rs9939609 in the Bangladeshi population that revealed significant association with T2DM and also imply that the connection between them may be mediated by mechanisms other than obesity and increased BMI which was supported by results found in most of the Asian populations. This study could be used as a foundation for future association studies involving larger populations.