학술논문
Primary structure of chicken muscle pyruvate kinase mRNA.
Document Type
Article
Author
Source
Proceedings of the National Academy of Sciences of the United States of America; June 1983, Vol. 80 Issue: 12 p3661-3665, 5p
Subject
Language
ISSN
00278424; 10916490
Abstract
We have determined the cDNA sequence corresponding to chicken muscle pyruvate kinase mRNA; the predicted coding region spans 529 amino acids and establishes the complete amino acid sequence for the vertebrate enzyme. We demonstrate that the level of mRNA for this enzyme is under developmental control and suggest a structural model for the protein kinase-mediated regulation of the mammalian liver isozyme. We report a method for the direct analysis of, and the preparation of cDNA probes from, mRNA which has been fractionated on methylmercury/agarose gels.