학술논문
A Rapid Purification Procedure for Glyceraldehyde 3-Phosphate Dehydrogenase from Bakers' Yeast
Document Type
Article
Source
Journal of Biological Chemistry; October 1972, Vol. 247 Issue: 19 p6277-6279, 3p
Subject
Language
ISSN
00219258; 1083351X
Abstract
A new procedure utilizing column chromatography has been devised for the purification of yeast glyceraldehyde 3-phosphate dehydrogenase. It yields enzyme of the highest specific activity in a shorter period of time and with a better yield than the traditional procedure. The isozyme isolated is the most abundant and most acidic of the set of isozymes previously identified.