학술논문
Feasibility of collection and analysis of microbiome data in a longitudinal randomized trial of community gardening
Document Type
Article
Author
Source
Future Microbiology; June 2020, Vol. 15 Issue: 8 p633-648, 16p
Subject
Language
ISSN
17460913; 17460921
Abstract
Aim:We explored the feasibility of collecting and analyzing human microbiome data in a longitudinal randomized controlled trial of community gardening. Methods & materials:Participants were randomly assigned to gardening (N = 8) or control (N = 8). Participants provided stool, mouth, hand and forehead microbiome samples at six timepoints. Analyses combined mixed models with Qiita output. Results:Participant satisfaction was high, with 75% of participants completing evaluations. While no microbial effects were statistically significant due to small sample size, the analysis pipeline utility was tested. Conclusion:Longitudinal collection and analysis of microbiome data in a community gardening randomized controlled trial is feasible. The analysis pipeline will be useful in larger studies for assessment of the pathway between microbiota, gardening and health outcomes.