학술논문

Flt‐3 ligand (FL) drives differentiation of rat bone marrow‐derived dendritic cells expressing OX62 and/or CD161 (NKR‐P1)
Document Type
Article
Source
Journal of Leukocyte Biology; June 2002, Vol. 71 Issue: 6 p941-949, 9p
Subject
Language
ISSN
07415400; 19383673
Abstract
Bone marrow‐derived dendritic cells (DC) of the rat have not been as well characterized as those from the mouse. Here, large quantities of bone marrow‐derived rat DC were generated when Flt‐3 ligand (FL) was used as an adjunct to granulocyte macrophage‐colony stimulating factor (GM‐CSF) and interleukin‐4 (IL‐4). These cells displayed a typical DC phenotype, expressing MHC class II, CD54, CD80, CD86, and CD11b/c. These DC also uniformly expressed low levels of CD161 and expressed OX62 in a bimodal distribution. Few cells were recovered from cultures grown without FL, and they failed to express OX62 or CD161. The DC generated with FL were more potent antigen‐presenting cells in mixed lymphocyte cultures than cells grown without FL, and among FL‐derived cells, the OX62+cells were slightly more stimulatory than OX62−cells. Thus, FL is a useful cytokine for obtaining large quantities of functional rat DC subsets in vitro.