학술논문
Europium‐Labeled Activity‐Based Probe through Click Chemistry: Absolute Serine Protease Quantification Using 153Eu Isotope Dilution ICP/MS
Document Type
Article
Author
Source
Angewandte Chemie. International Edition; April 2012, Vol. 51 Issue: 14 p3358-3363, 6p
Subject
Language
ISSN
14337851; 15213773
Abstract
Click and analyze: The titled probe was synthesized by conjugating a sulfonyl fluoride and azido unit using click chemistry to give SF‐Eu, which can react specifically with serine (Ser) in the active site of serine protease (SP). Combination of the method with 153Eu‐isotope dilution ICP/MS enables absolute protein quantification of active SPs in biological samples using only one 153Eu(NO3)3isotopic standard.