부산대학교 도서관

A major consideration in designing dental implants is the creation of a surface that provides strong attachment between the implant and bone, connective tissue, or epithelium. In addition, it is important to inhibit the adherence of oral bacteria on titanium surfaces exposed to the oral cavity to maintain plaquefree implants. Previous in vitrostudies have shown that titanium implant surfaces coated with titanium nitride TiN reduced bacterial colonization compared to other clinically used implant surfaces. The aim of the present study was to examine the support of fibroblast growth by a TiN surface that has antimicrobial characteristics. Mouse fibroblasts were cultured on smooth titanium discs that were either magnetronsputtered with a thin layer of titanium nitride, thermal oxidized, or modified with laser radiation using a NdYAG laser. The resulting surface topography was examined by scanning electron microscopy SEM, and surface roughness was estimated using a twodimensional contact stylus profilometer. A protein assay BCA assay and a colorimetric assay to examine fibroblast metabolism MTT were used. Cellular morphology and cell spreading were analyzed using SEM and fluorescence microscopy. Fibroblasts on oxidized titanium surfaces showed a more spherical shape, whereas cells on lasertreated titanium and on TiN appeared intimately adherent to the surface. The MTT activity and total protein were significantly increased in fibroblasts cultured on titanium surfaces coated with TiN compared to all other surface modifications tested. This study suggests that a titanium nitride coating might be suitable to support tissue growth on implant surfaces. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 64A: 591–599, 2003