학술논문
DiO-C3 -(5) and DiS-C3 -(5): Interactions with RBC, ghosts and phospholipid vesicles
Document Type
Article
Author
Source
Journal of Membrane Biology; February 1981, Vol. 59 Issue: 1 p1-11, 11p
Subject
Language
ISSN
00222631; 14321424
Abstract
Summary The experiments presented below compared the interaction of diO-C3 -(5) and diS-C3 -(5) with erythrocytes, erythrocyte ghosts and phospholipid vesicles derived from erythrocyte membranes. The results confirm earlier reports of diS-C3 -(5) dimerization in the presence of hemoglobin and of dye aggregate formation in erythrocyte suspensions. DiO-C3 -(5), on the other hand, binds to vesicles and ghosts freed of hemoglobin in a potential-dependent manner but without forming dye aggregates. The two dyes bind to the different preparations in similar proportions, but diS-C3 -(5) is bound in amounts 3–40 times greater depending on the degree of polarization.