부산대학교 도서관

Methacrylated gelatin films with in situ-generated TiO2 nanoparticles containing varying weight percentages of gelatin (0 %, 0.5 %, 1 %, 2 % and 4 %) were successfully prepared as novel biomaterials. 1H-NMR spectroscopy confirmed their methacrylation with a 79 % degree of substitution. TiO2 nanoparticles were uniformly distributed in the films with the average particle size increasing from 85 to 130 nm in proportion to an increase in TiO2 concentration from 0.5 to 4 wt%. The water absorption of various gelatin methacrylamide/TiO2 films was in the range of 471–758 %, which was enough to prevent wound beds from exudates accumulation. And in vitro degradation test in PBS showed that the three-dimensional structure of all samples basically remained unchanged although more than or nearly half the mass of specimens decreased after 4 weeks’ degradation, and the pH levels of all sample solutions were maintained in an adequate range of 6.5–7.4 for cell and tissue growth during the whole process. The antibacterial activities of the films against E. coli and S. aureus were measured via a shake flask test and demonstrated good performance after the importation of TiO2 nanoparticles. Cytotoxicity testing revealed that all films had no cytotoxicity and showed favorable adherence in the presence of L929 cells. The results suggest that hybrid hydrogel films hold potential for antibacterial wound dressing and tissue engineering scaffold applications.