부산대학교 도서관

Oxidative stress induces cell senescence and aging. Cell senescence is an irreversible cell cycle arrest mechanism responsible for various pathological diseases and aging. Cerium oxide nanoparticles (CeO2 NPs) have been found to have anti-oxidant effects with a capacity to scavenge free radicals. With this background, we aimed to investigate the senolytic property of CeO2 NPs on mouse embryonic fibroblasts (NIH3T3). NIH3T3 cells were exposed to CeO2 NPs with or without hydrogen peroxide (H2O2). The senolytic effect of CeO2 NPs was evaluated by measuring reactive oxygen species (ROS) production, lipid peroxidation (LPO), β-galactosidase, cell cycle phase analysis, and expression of p21, p38, p53, and NF-кB genes. EC50 of CeO2 NPs significantly reduced the β-galactosidase activity in NIH3T3 cells without apparent cytotoxicity and generation of ROS. Also, it decreased LPO, and expression of IL-6. The number of cells increased significantly, indicating that CeO2 NPs can reverse cell senescence and cell cycle arrest in the S phase. Also, p21, p38, p53, and NF-кB gene expressions were significantly down-regulated. CeO2 NPs as a senolytic or inhibitor of senescence can attenuate the senescence-inducing signal transduction pathways, i.e., p38/ NF-кB and p53/p21. It reduced the production of ROS and LPO and the expression of IL-6.Graphic Abstract: