학술논문
Tenascin-C is synthesised and secreted by megakaryocytes, whose adherence to intact tenascin is mediated by the integrin subunit α1
Document Type
Original Paper
Source
Comparative Haematology International. September 1998 8(3):142-149
Subject
Language
English
ISSN
0938-7714
1433-2973
1433-2973
Abstract
Bone marrow megakaryocytes reside on a complex extracellular matrix, produced by stromal cells, as well as by megakaryocytes themselves. Using immunofluorescence microscopy we have determined the distribution of tenascin-C in guinea-pig bone marrow, finding that it has a prominent fibrillar distribution in association with megakaryocytes. Western blot analysis of CHRF-288 cells, a human megakaryocytic cell line and isolated purified guinea-pig megakaryocytes, with polyclonal and monoclonal antibodies, demonstrate the presence of three tenascin isoforms (210, 220 and 250 kDa). Immunoprecipitation of radiolabelled isolated guinea-pig megakaryocytes cultured on a type I collagen gel, demonstrate that these three tenascin isoforms were present in mega-karyocytes, whereas only two isoforms were secreted into the media (210 and 220 kDa). Analysis of rat bone marrow by in situ hybridisation reveals that transcripts for tenascin-C are present in megakaryocytes. Integrin regulation of CHRF cell adhesion to intact tenascin was shown to be mediated solely by theβ1 integrin.