학술논문
Differences in PPD- and mitogen-induced T-cell activation marker expression characterize immunopathology in acute tuberculosis patients
Document Type
Brief Communication
Author
Acheampong, Isaac; Minadzi, Difery; Laing, Edwin F.; Frimpong, Michael; Vivekanandan, Monika M.; Yeboah, Augustine; Adankwah, Ernest; Aniagyei, Wilfred; Arthur, Joseph F.; Lamptey, Millicent; Abass, Mohammed K.; Kumbel, Francis; Osei-Yeboah, Francis; Gawusu, Amidu; Debrah, Linda Batsa; Owusu, Dorcas O.; Debrah, Alexander; Mayatepek, Ertan; Seyfarth, Julia; Phillips, Richard O.; Jacobsen, Marc
Source
European Journal of Clinical Microbiology & Infectious Diseases. 43(3):611-616
Subject
Language
English
ISSN
0934-9723
1435-4373
1435-4373
Abstract
Impaired T-cell responses to mitogens and high T-cell activation marker (TAM) expression on Mycobacterium tuberculosis–specific T-cells characterize immunopathology in patients with tuberculosis (TB). In a study of patients with TB (n = 60) and asymptomatic contacts (controls, n = 37), we found that TB patients had higher CD38+ T-cell proportions specific for M. tuberculosis protein (PPDMtb ), yet total proportions of PPDMtb -specific T-cells were comparable. Notably, both activated (CD38+ ) and total IFN-γ+ T-cells from TB patients had lower mitogen (phytohemagglutinin, PHA)-induced responses. This impaired mitogen response improved the classification efficacy of the TAM-TB assay, especially employing the PPD/PHA-induced T-cell ratio.