학술논문
Mitochondrial variant enrichment from high-throughput single-cell RNA sequencing resolves clonal populations
Document Type
Original Paper
Author
Miller, Tyler E.; Lareau, Caleb A.; Verga, Julia A.; DePasquale, Erica A. K.; Liu, Vincent; Ssozi, Daniel; Sandor, Katalin; Yin, Yajie; Ludwig, Leif S.; El Farran, Chadi A.; Morgan, Duncan M.; Satpathy, Ansuman T.; Griffin, Gabriel K.; Lane, Andrew A.; Love, J. Christopher; Bernstein, Bradley E.; Sankaran, Vijay G.; van Galen, Peter
Source
Nature Biotechnology: The Science and Business of Biotechnology. 40(7):1030-1034
Subject
Language
English
ISSN
1087-0156
1546-1696
1546-1696
Abstract
The combination of single-cell transcriptomics with mitochondrial DNA variant detection can be used to establish lineage relationships in primary human cells, but current methods are not scalable to interrogate complex tissues. Here, we combine common 3′ single-cell RNA-sequencing protocols with mitochondrial transcriptome enrichment to increase coverage by more than 50-fold, enabling high-confidence mutation detection. The method successfully identifies skewed immune-cell expansions in primary human clonal hematopoiesis.
Clonal dynamics are inferred from mitochondrial variants in primary human cells.
Clonal dynamics are inferred from mitochondrial variants in primary human cells.