학술논문
An alternative method for Staphylococcus aureus DNA isolation
Document Type
article
Author
Source
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. April 2008 60(2)
Subject
Language
English
ISSN
0102-0935
Abstract
This study describes a rapid procedure for the isolation of genomic DNA from Staphylococcus aureus that yielded a good amount of high quality DNA for the amplification of staphylococcal enterotoxins genes (A, B, C, D, and E) and the TSST-1 gene as well as enzymatic restriction (HaeIII) from environmental isolates. With this method, it was possible to detect these genes in a sample containing as little as 10(5) cells with positive PCR reactions obtained from approximately 10pg of DNA in a final reaction volume of 25µl.