학술논문
Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus
Document Type
article
Source
Revista da Sociedade Brasileira de Medicina Tropical. October 2013 46(5)
Subject
Language
English
ISSN
0037-8682
Abstract
Introduction Herpes simplex virus (HSV) and varicella zoster virus (VZV) are responsible for a variety of human diseases, including central nervous system diseases. The use of polymerase chain reaction (PCR) techniques on cerebrospinal fluid samples has allowed the detection of viral DNA with high sensitivity and specificity. Methods Serial dilutions of quantified commercial controls of each virus were subjected to an in-house nested-PCR technique. Results The minimum detection limits for HSV and VZV were 5 and 10 copies/µL, respectively. Conclusions The detection limit of nested-PCR for HSV and VZV in this study was similar to the limits found in previous studies.