학술논문
Immunoprofiles associated with controlled human malaria infection and naturally acquired immunity identify a shared IgA pre-erythrocytic immunoproteome
Document Type
article
Author
Berry, Andrea A; Obiero, Joshua M; Travassos, Mark A; Ouattara, Amed; Coulibaly, Drissa; Adams, Matthew; de Assis, Rafael Ramiro; Jain, Aarti; Taghavian, Omid; Sy, Andrew; Nakajima, Rie; Jasinskas, Algis; Laurens, Matthew B; Takala-Harrison, Shannon; Kouriba, Bourema; Kone, Abdoulaye K; Doumbo, Ogobara K; Sim, B Kim Lee; Hoffman, Stephen L; Plowe, Christopher V; Thera, Mahamadou A; Felgner, Philip L; Lyke, Kirsten E
Source
npj Vaccines. 6(1)
Subject
Language
Abstract
Knowledge of the Plasmodium falciparum antigens that comprise the human liver stage immunoproteome is important for pre-erythrocytic vaccine development, but, compared with the erythrocytic stage immunoproteome, more challenging to classify. Previous studies of P. falciparum antibody responses report IgG and rarely IgA responses. We assessed IgG and IgA antibody responses in adult sera collected during two controlled human malaria infection (CHMI) studies in malaria-naïve volunteers and in 1- to 6-year-old malaria-exposed Malian children on a 251 P. falciparum antigen protein microarray. IgG profiles in the two CHMI groups were equivalent and differed from Malian children. IgA profiles were robust in the CHMI groups and a subset of Malian children. We describe immunoproteome differences in naïve vs. exposed individuals and report pre-erythrocytic proteins recognized by the immune system. IgA responses detected in this study expand the list of pre-erythrocytic antigens for further characterization as potential vaccine candidates.