학술논문

Protocol for vision transformer-based evaluation of drug potency using images processed by an optimized Sobel operator
Document Type
article
Source
STAR Protocols. 4(2)
Subject
Information and Computing Sciences
Medicinal and Biomolecular Chemistry
Chemical Sciences
Substance Misuse
Drug Abuse (NIDA only)
Neurosciences
Prevention
5.1 Pharmaceuticals
Development of treatments and therapeutic interventions
Biotechnology and bioengineering
Cancer
Computer sciences
High Throughput Screening
Microscopy
Language
Abstract
Conventional approaches for screening anticancer drugs rely on chemical reactions, which are time consuming, labor intensive, and costly. Here, we present a protocol for label-free and high-throughput assessment of drug efficacy using a vision transformer and a Conv2D. We describe the steps for cell culture, drug treatment, data collection, and preprocessing. We then detail the building of deep learning models and their use to predict drug potency. This protocol can be adapted for screening chemicals that affect the density or morphological features of cells. For complete details on the use and execution of this protocol, please refer to Wang et al.1.