학술논문
Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution
Document Type
article
Author
Source
Nature Methods. 16(6)
Subject
Language
Abstract
We designed an epi-illumination SPIM system that uses a single objective and has a sample interface identical to that of an inverted fluorescence microscope with no additional reflection elements. It achieves subcellular resolution and single-molecule sensitivity, and is compatible with common biological sample holders, including multi-well plates. We demonstrated multicolor fast volumetric imaging, single-molecule localization microscopy, parallel imaging of 16 cell lines and parallel recording of cellular responses to perturbations.