학술논문

Antiquaquibacter oligotrophicus gen. nov., sp. nov., a novel oligotrophic bacterium from groundwater.
Document Type
article
Source
International Journal of Systematic and Evolutionary Microbiology. 73(12)
Subject
Microbiology
Biological Sciences
Genetics
Phylogeny
RNA
Ribosomal
16S
Base Composition
Fatty Acids
Sequence Analysis
DNA
DNA
Bacterial
Bacterial Typing Techniques
Bacteria
Actinomycetales
Amino Acids
Groundwater
Antiquaquibacter oligotrophicus
Microbacteriaceae
polyphasic description.
polyphasic description
Evolutionary Biology
Medical Microbiology
Evolutionary biology
Language
Abstract
In this study, a Gram-stain-positive, non-motile, oxidase- and catalase-negative, rod-shaped, bacterial strain (SG_E_30_P1T) that formed light yellow colonies was isolated from a groundwater sample of Sztaravoda spring, Hungary. Based on 16S rRNA phylogenetic and phylogenomic analyses, the strain was found to form a distinct linage within the family Microbacteriaceae. Its closest relatives in terms of near full-length 16S rRNA gene sequences are Salinibacterium hongtaonis MH299814 (97.72 % sequence similarity) and Leifsonia psychrotolerans GQ406810 (97.57 %). The novel strain grows optimally at 20-28 °C, at neutral pH and in the presence of NaCl (1-2 w/v%). Strain SG_E_30_P1T contains MK-7 and B-type peptidoglycan with diaminobutyrate as the diagnostic amino acid. The major cellular fatty acids are anteiso-C15 : 0, iso-C16 : 0 and iso-C14 : 0, and the polar lipid profile is composed of diphosphatidylglycerol and phosphatidylglycerol, as well as an unidentified aminoglycolipid, aminophospholipid and some unidentified phospholipids. The assembled draft genome is a contig with a total length of 2 897 968 bp and a DNA G+C content of 65.5 mol%. Amino acid identity values with it closest relatives with sequenced genomes of