학술논문
Nucleic acid biomarkers of immune response and cell and tissue damage in children with COVID-19 and MIS-C
Document Type
article
Author
Loy, Conor J; Sotomayor-Gonzalez, Alicia; Servellita, Venice; Nguyen, Jenny; Lenz, Joan; Bhattacharya, Sanchita; Williams, Meagan E; Cheng, Alexandre P; Bliss, Andrew; Saldhi, Prachi; Brazer, Noah; Streithorst, Jessica; Suslovic, William; Hsieh, Charlotte J; Bahar, Burak; Wood, Nathan; Foresythe, Abiodun; Gliwa, Amelia; Bhakta, Kushmita; Perez, Maria A; Hussaini, Laila; Anderson, Evan J; Chahroudi, Ann; Delaney, Meghan; Butte, Atul J; DeBiasi, Roberta L; Rostad, Christina A; De Vlaminck, Iwijn; Chiu, Charles Y
Source
Cell Reports Medicine. 4(6)
Subject
Language
Abstract
Differential host responses in coronavirus disease 2019 (COVID-19) and multisystem inflammatory syndrome in children (MIS-C) remain poorly characterized. Here, we use next-generation sequencing to longitudinally analyze blood samples from pediatric patients with COVID-19 or MIS-C across three hospitals. Profiling of plasma cell-free nucleic acids uncovers distinct signatures of cell injury and death between COVID-19 and MIS-C, with increased multiorgan involvement in MIS-C encompassing diverse cell types, including endothelial and neuronal cells, and an enrichment of pyroptosis-related genes. Whole-blood RNA profiling reveals upregulation of similar pro-inflammatory pathways in COVID-19 and MIS-C but also MIS-C-specific downregulation of T cell-associated pathways. Profiling of plasma cell-free RNA and whole-blood RNA in paired samples yields different but complementary signatures for each disease state. Our work provides a systems-level view of immune responses and tissue damage in COVID-19 and MIS-C and informs future development of new disease biomarkers.