학술논문
Circulating trophoblast cell clusters for early detection of placenta accreta spectrum disorders.
Document Type
article
Author
Afshar, Yalda; Dong, Jiantong; Zhao, Pan; Li, Lei; Wang, Shan; Zhang, Ryan Y; Zhang, Ceng; Yin, Ophelia; Han, Christina S; Einerson, Brett D; Gonzalez, Tania L; Zhang, Huirong; Zhou, Anqi; Yang, Zhuo; Chou, Shih-Jie; Sun, Na; Cheng, Ju; Zhu, Henan; Wang, Jing; Zhang, Tiffany X; Lee, Yi-Te; Wang, Jasmine J; Teng, Pai-Chi; Yang, Peng; Qi, Dongping; Zhao, Meiping; Sim, Myung-Shin; Zhe, Ruilian; Goldstein, Jeffrey D; Williams, John; Wang, Xietong; Zhang, Qingying; Platt, Lawrence D; Zou, Chang; Pisarska, Margareta D; Tseng, Hsian-Rong; Zhu, Yazhen
Source
Nature communications. 12(1)
Subject
Language
Abstract
Placenta accreta spectrum (PAS) is a high-risk obstetrical condition associated with significant morbidity and mortality. Current clinical screening modalities for PAS are not always conclusive. Here, we report a nanostructure-embedded microchip that efficiently enriches both single and clustered circulating trophoblasts (cTBs) from maternal blood for detecting PAS. We discover a uniquely high prevalence of cTB-clusters in PAS and subsequently optimize the device to preserve the intactness of these clusters. Our feasibility study on the enumeration of cTBs and cTB-clusters from 168 pregnant women demonstrates excellent diagnostic performance for distinguishing PAS from non-PAS. A logistic regression model is constructed using a training cohort and then cross-validated and tested using an independent cohort. The combined cTB assay achieves an Area Under ROC Curve of 0.942 (throughout gestation) and 0.924 (early gestation) for distinguishing PAS from non-PAS. Our assay holds the potential to improve current diagnostic modalities for the early detection of PAS.