학술논문
Identification of Vaccine Candidates Against Serogroup B Meningococcus by Whole-Genome Sequencing
Document Type
Academic Journal
Author
Pizza, Mariagrazia; Scarlato, Vincenzo; Masignani, Vega; Giuliani, Marzia Monica; Aricò, Beatrice; Comanducci, Maurizio; Jennings, Gary T.; Baldi, Lucia; Bartolini, Erika; Capecchi, Barbara; Galeotti, Cesira L.; Luzzi, Enrico; Manetti, Roberto; Marchetti, Elisa; Mora, Marirosa; Nuti, Sandra; Ratti, Giulio; Santini, Laura; Savino, Silvana; Scarselli, Maria; Storni, Elisa; Zuo, Peijun; Broeker, Michael; Hundt, Erika; Knapp, Bernard; Blair, Eric; Mason, Tanya; Tettelin, Hervé; Hood, Derek W.; Jeffries, Alex C.; Saunders, Nigel J.; Granoff, Dan M.; Venter, J. Craig; Moxon, E. Richard; Grandi, Guido; Rappuoli, Rino
Source
Science. Mar 10, 2000 287(5459):1816-1820
Subject
Language
English
ISSN
0036-8075
Abstract
Neisseria meningitidis is a major cause of bacterial septicemia and meningitis. Sequence variation of surface-exposed proteins and cross-reactivity of the serogroup B capsular polysaccharide with human tissues have hampered efforts to develop a successful vaccine. To overcome these obstacles, the entire genome sequence of a virulent serogroup B strain (MC58) was used to identify vaccine candidates. A total of 350 candidate antigens were expressed in Escherichia coli, purified, and used to immunize mice. The sera allowed the identification of proteins that are surface exposed, that are conserved in sequence across a range of strains, and that induce a bactericidal antibody response, a property known to correlate with vaccine efficacy in humans.