학술논문
Reduced susceptibility to biocides in Acinetobacter baumannii: association with resistance to antimicrobials, epidemiological behaviour, biological cost and effect on the expression of genes encoding porins and efflux pumps
Document Type
Academic Journal
Author
Fernández-Cuenca, Felipe; Tomás, María; Caballero-Moyano, Francisco-Javier; Bou, Germán; Martínez-Martínez, Luis; Vila, Jordi; Pachón, Jerónimo; Cisneros, José-Miguel; Rodríguez-Baño, Jesús; Pascual, Álvaro; Garnacho, José; Gutierrez-Pizarraya, Antonio; Márquez-Vácaro, Juan Antonio; Cano, María Eliecer; Fariñas, M. Carmen; Sánchez-Porto, Antonio; Meruendano, Gloria Esteban; Barbeyto-Vales, Luis; Casas-Ciria, Javier; Vallejo, Luis; Fernández-Pérez, Begona; Villar-Chao, José Carlos; Padilla-Ortega, Belén; Cercenado-Mansilla, Emilia; García-Irure, José Javier; del Arco Jiménez, Alfonso; Gimeno-Cardona, Concepción; Valía, Juan Carlos; Tormo-Palop, Núria; Abril, Vicente; Rifa, Josefina; Martinez-Garcia, Maria Jesus; Vilaró-Pujals, Joseph; Aguirre, Marian Navarro; Vilamala, Ana; Jiménez-Alfaro, José Antonio; Reviejo-Jaca, Carlos; Casanova, Pilar Marín; Guerreo, Francisca; Shaw, Evelyn; Plasencia, Virginia; Nebreda-Mayoral, Teresa; Fernández-Calavia, María José; de Cruz, Susana García; Aldea-Mansilla, Carmen; de Lucas, Esperanza Merino; Zorraquino, Alfredo; Reus-Bañuls, Sergio; Garduno-Eseverri, Eugenio; Sánchez, Luis López; Fleites-Gutiérrez, Ana; Rodríguez-Guardado, Azucena; Moreno, Alfonso; García-Arenzana Anguera, José María; López-Palmero, Serafín; Rodríguez-Maresca, Manuel; García-Garrote, Fernando; Varela-Otero, José; del Pilar Alonso, María; Vidal-Verdú, Elisa; Rodríguez-López, Fernando; Pardo-Sánchez, Fernanda; Ferrer-Vizoso, E.; Regueiro-Garcia, B.; Gurgui, Mercé; Pericas, Roser; Pomar, Virginia; Olaechea-Astigarraga, Pedro María; Ayarza-Igartua, Rafael; Maciá-Romero, María Dolores; de Gopegui-Bordes, Enrique Ruiz; Sánchez-Romero, María Isabel; García-Mata, Jesús; Goyanes, María José; Morales-Mateos, Cristina; Hernández-Quero, José; Escobar-Lara, Trinidad; Ballester-Bastardie, Frederic; Iftimie, Simona; Pujol-Bajador, Isabel; Galán-Navarro, María Isabel; Cádiz-Gurrea, María Luz; Amores-Antequera, Carmen; Gómez, Montserrat; Cantudo, Purificación; Martí-Salas, Carmina; Cuquet-Peragosa, Jordi; Moreno-Flores, Antonio; Anibarro-García, Luis; Hernando-Real, Susana; Carrero-González, Pablo A.; Pallarés-González, María Angeles; Rodríguez-Fernández, Sergio; Pujol-Rojo, Miquel; Tubau, Fe; Nuno-Alvarez, Enrique; Ortega-Torres, María; Giner-Almaraz, Salvador; Roca-Castelló, María Rosa; Castillo, Manuela; Hortelano, Elena; Chaves-Sánchez, Fernando; García-Reyne, Ana; Horcajada-Gallego, Juan Pablo; Segura, Concha; Sierra-Dorado, Gema; Yano-Escudero, Raquel; Dorta-Hung, María Elena; del Rosario Q, Cristóbal
Source
Journal of Antimicrobial Chemotherapy. Dec 01, 2015 70(12):3222-3229
Subject
Language
English
ISSN
0305-7453
Abstract
OBJECTIVES: The objective of this study was to analyse whether there is an association between reduced susceptibility to biocides in Acinetobacter baumannii and (i) antimicrobial resistance (co-resistance), (ii) prevalent (epidemic) clones, (iii) changes in the fitness or (iv) expression of genes related to efflux pumps and porins. METHODS: Susceptibility to biocides and antimicrobials was determined in 49 clonally unrelated isolates of A. baumannii. Biological cost, in terms of mean generation time, was determined by spectrophotometry. Quantitative real-time RT–PCR was used to determine the relative expression of genes encoding several efflux pumps and porins. RESULTS: Reduced susceptibility to chlorhexidine digluconate, benzalkonium chloride and Irgasan was associated with resistance to aminoglycosides, tetracycline and ciprofloxacin (P < 0.05). The MICs of carbapenems, aminoglycosides, doxycycline and ciprofloxacin for isolate Ab70 (epidemic clone) exposed to these biocides increased by ≥2 dilutions. Reduced susceptibility to Orsan was more frequent among prevalent clones than non-prevalent clones (P < 0.05). Mean generation times for Ab70 before and after exposure to benzalkonium chloride were 57.8 and 78.1 min, respectively (P = 0.02). Relative expression of abeS and adeB was increased in Ab46 and Ab70 after exposure to chlorhexidine digluconate, but was decreased for ompA and carO after exposure to Irgasan. CONCLUSIONS: Reduced susceptibility to biocides is associated with co-resistance to carbapenems, aminoglycosides, tetracycline and ciprofloxacin. Reduced susceptibility to Orsan may be a marker of prevalent clones. Acquisition of reduced susceptibility to benzalkonium chloride has a biological cost. Exposure to biocides affects the relative expression of genes related to some efflux pump genes (increased expression) or porins (reduced expression).