부산대학교 도서관

Oxidative stress can be caused by impaired balance between free radical and anti-oxidant defense mechanism and leads to destruction of cellular structure and tissue damage, followed by ultimately affecting human health and lifespan. In this regard many studies have focused on the development of antioxidant that can eradicate free radicals including reactive oxygen species. This study was designed to elucidate the antioxidant activity of prenylated chalcone compound named 7,9,2',4'-Tetrahydroxy-8isopentenyl-5methoxychalcone (THIPMC) which can be separated from roots of Sophora flavescens. Data from in vitro assays represented that THIPMC has strong anti-oxidative capacity on the DPPH radicals and superoxide anions similar to that of ascorbic acid. In accordance with in vitro results, further studies using Caenorhabditis elegans also have shown that THIPMC could modulate the antioxidant defense mechanism in vivo. In this work, THIPMC increased the survival rate of nematode under oxidative stress condition induced by rotenone. Moreover, THIPMC treatment could attenuated intracellular ROS by modulating activities of antioxidant enzymes such as superoxide dismutase and catalase. Also, expressions of SOD-3 and DAF-16 were up-regulated in presence of THIPMC. These results indicate that THIPMC can be a great candidate of valuable antioxidant agent.