학술논문
소나무재선충 유인물질에 의한 소나무재선충병 진단키트의 개발 / Development of Diagnosis Kit for Pine Wilt Disease, Using Attractants to Bursaphelenchus xylophilus (Tylenchida: Aphenlenchoididae)
Document Type
Dissertation/ Thesis
Author
Source
Subject
Language
Korean
Abstract
소나무의 고사가 진행되는 초기에 소나무재선충에 의한 감염여부를 신속하게 진단하기 위하여 소나무류에서 휘발되는 18종류 휘발물질의 소나무재선충에 대한 유인력을 실험하고, 유인력이 우수한 물질로 소나무재선충병 진단키트를 개발하였다. 하루 동안 절식시킨 증식형 소나무재선충은 α-pinene, β-pinene 및 camphor에 많이 유인되었으나, 절식시키지 않은 증식형 소나무재선충은 α-pinene과 β-pinene에 유인 효과가 있었다. 그러나 분산형 유충에 대해서는 절식여부와 관계없이 α-pinene, β-pinene, camphor의 유인력이 높았다. 이들 세 물질로써 소나무재선충 감염여부 검정용 진단키트를 만들어 인공 용실과 감염 고사목에서 소나무재선충에 대한 유인력을 실험하였다. 인공 용실에서 진단키트에 휘발성 물질을 처리한 24시간 후 보다는 48시간 후의 유인력이 더 좋았고, 휘발성 물질을 filter paper에 처리했을 때보다는 cotton ball에 처리했을 때 유인 효과가 더 높았다. 감염목에서 진단키트의 cotton ball에 휘발성물질을 처리하여 유인력을 실험한 결과 8시간 후부터 소나무재선충이 유인되었으며, camphor의 유인력이 가장 높았다. α-pinene, β-pinene, camphor는 소나무재선충에 대해서는 유인 효과가 있었으나 어리소나무재선충이나 부식성 선충에 대해서는 유인 효과가 없었다.
A kit was developed to diagnose the pine wilt disease, using volatiles attractive to pine wood nematode (PWN) Bursaphelenchus xylophilus (Tylenchida: Aphenlenchoididae). Attractiveness test for 18 volatiles emitting from pine tree species on Petri-dish showed that α-pinene, β-pinene, and camphor attracted significantly higher number of both reproductive and dispersal types of PWN, irrespective of starvation. However, the 1-day starved reproductive PWN was more sensitive to those volatiles than non-starved one. The diagnosis kits using the three chemicals attracted higher number of PWN in artificial pupal chamber, compared with check treatment. The cotton ball as a carrier of volatiles was more effective than filter paper. The pine logs (from pine trees infected with PWN and died) test using cotton ball as a carrier showed that diagnosis kit baited with camphor attracted significantly more PWN from 8 h after kit insertion into the log than check treatment. And the camphor diagnosis kit was significantly more attractive to PWN than α-pinene and β-pinene kits. Even though there were no statistical differences in PWN attractiveness among α-pinene, β-pinene, and check treatment in pine log, these two chemicals attracted 22 to 47 times more PWN than check treatment. The diagnosis kit baited with camphor did not attract B. mucronatus and saprophagous nematodes. This selective attractiveness of camphor to PWN was confirmed on Petri-dish test in the laboratory. This diagnosis kit using camphor may be applied to pine trees in the forest and will help to find out the PWN from dead or dying pine trees in easy and quick way.
A kit was developed to diagnose the pine wilt disease, using volatiles attractive to pine wood nematode (PWN) Bursaphelenchus xylophilus (Tylenchida: Aphenlenchoididae). Attractiveness test for 18 volatiles emitting from pine tree species on Petri-dish showed that α-pinene, β-pinene, and camphor attracted significantly higher number of both reproductive and dispersal types of PWN, irrespective of starvation. However, the 1-day starved reproductive PWN was more sensitive to those volatiles than non-starved one. The diagnosis kits using the three chemicals attracted higher number of PWN in artificial pupal chamber, compared with check treatment. The cotton ball as a carrier of volatiles was more effective than filter paper. The pine logs (from pine trees infected with PWN and died) test using cotton ball as a carrier showed that diagnosis kit baited with camphor attracted significantly more PWN from 8 h after kit insertion into the log than check treatment. And the camphor diagnosis kit was significantly more attractive to PWN than α-pinene and β-pinene kits. Even though there were no statistical differences in PWN attractiveness among α-pinene, β-pinene, and check treatment in pine log, these two chemicals attracted 22 to 47 times more PWN than check treatment. The diagnosis kit baited with camphor did not attract B. mucronatus and saprophagous nematodes. This selective attractiveness of camphor to PWN was confirmed on Petri-dish test in the laboratory. This diagnosis kit using camphor may be applied to pine trees in the forest and will help to find out the PWN from dead or dying pine trees in easy and quick way.