부산대학교 도서관

Formation of a wide array of inorganic materials can be mediated by the biomolecule in the biosphere, such as silica cell wall, bone, shells, lenses, and magnetic particles. Recently, in vitro experiments with biomolecule such as peptides, RNA, and DNA have shown that templating by these macromolecules can yield a variety of materials under mild reaction conditions. In this research, a disulfide-bond constrained peptide (STB1: -CHKKPSKSC-) cognitive of TiO2 was screened, synthesized and demonstrated to catalyze the formation of TiO2 nanoparticles in thermodynamically unfavorable condition. In addition, STB1 was modified to be used for the biomineralization. The effects of peptide concentration, salt concentration and pH value to the TiO2 precipitation were investigated. All the synthesized nanoparticles were subsequently characterized by scanning electron microscope (SEM); transmit electron microscope (TEM), energy dispersive spectrometer (EDS) and X-Ray diffraction (XRD). Formation of spherical nanoparticles was found to take place above a critical concentration of STB1. A RRIL tail which was found in nature was added at the C-terminal of the binding peptide and showed a significant enhancement to the inducing activity. The interaction between the peptide and precursor is electrostatic interaction and can be adjusted by varying the salt concentration and pH value of the solution. Furthermore, the particle size was clearly dependent on the specific activity of the peptide at the time of particle formation. This study provides interesting insights to understanding biomimetic synthesis of inorganic materials, hence potentially being harnessed for controlling nanoscale structures.