학술논문
식물면역활성제를 처리한 발아현미가 흰쥐의 비만억제에 미치는 영향 / Anti-obesity Effect of Germinated Brown Rice Treated with an Elicitor in Sprague-Dawley Rat
Document Type
Dissertation/ Thesis
Source
Subject
Language
Korean
Abstract
식물면역활성제를 식물에 접촉하면 식물은 자기방어를 하기 위하여 페놀성 화합물 등 2차대사산물을 생성하고 세포벽을 두껍게 한다. 본 연구에서는 키토산과 소금을 이용한 발아현미에 대한 유용성분과 생리활성을 측정하였으며, 키토산처리 발아현미 섭취가 흰쥐의 비만억제에 미치는 영향을 조사하였다. 시료는 0.03% 키토산과 0.05% 천일염을 사용하여 재배한 발아현미(CGBR)와 증류수로 재배한 발아현미(GBR) 두 종류이다. 유용성분으로는 페놀성화합물, 식이섬유, GABA, phytic acid 등을 측정하였는데 CGBR이 GBR보다 모두 높게 측정되었다. 이중 수용성식이섬유의 함량은 CGBR와 GBR간에 차이가 없었으나 불용성식이섬유의 함량은 CGBR와 GBR간에 차이를 나타냈다(CGBR: 8.1%, GBR: 6.9%). 또한 식물성분에서 항산화활성의 발현에 관여하는 페놀 화합물의 함량은 CGBR에서 GBR보다 높은 함량(CGBR: 92.1 mg/100g, GBR: 74.5 mg/100g)을 나타내었으며, GABA(ϒ-aminobutyric acid)함량에서도 동일한 효과를 나타냈다(CGBR: 1882.2 nmol/g, GBR: 1260.0 nmol/g). Phytic acid 함량은 CGBR가 23.9%로서 GBR의 2배로 측정되었다. Lipase 저해활성과 DPPH 라디칼 소거능을 측정해보았는데 Lipase 저해활성이 CGBR>GBR>BR의 순서였다. DPPH 라디칼 소거능은 GBR과 CGBR에서 차이를 보이지는 않지만 두 실험구 모두 추출물의 농도에 따라 소거능이 증가하였다. 우리는 키토산 처리 발아현미의 비만억제효과를 보기 위하여 수컷 흰쥐를 5개 집단으로 나누어 실험에 이용하였으며 실험기간은 총 4주였다. (Normal: 일반식이, n=8; Control: 고지방식이대조군, n=8; BR: 현미첨가 고지방식이, n=8; GBR: 발아현미첨가 고지방식이, n=8; CGBR: 키토산처리발아현미첨가 고지방식이, n=8) 4주동안 체중은 정상적으로 증가하였는데 CGBR의 체중증가량이 Control 집단보다 유의적으로 낮았다. (p
Elicitors are defined as substances that induce defence responses in plants, which include enhanced synthesis of secondary metabolites such as phenolic compounds and cell wall thickening. This study was to investigate the changes of physiologically active components in germinated brown rice treated with chitosan (CGBR) and its anti-obesity effect in Sprague-Dawley rat. Samples were prepared from the germinated brown rice treated with 0.03% chitosan containing 0.05% solar salts (CGBR) and with distilled water (GBR). Contents of physiologically active compounds such as total phenolic compounds, dietary fiber, ϒ-aminobutyric acid (GABA), and phytic acid in CGBR were significantly higher than those of GBR. Total phenolic compounds (92.1 mg/100g) in CGBR were higher than that (74.5 mg/100g) of GBR. GABA content (1882.2 nmol/g) in CGBR was about 1.5 fold higher than that (1260.0 nmol/g) of GBR. Phytic acid content (23.9 mg/g) of CGBR was higher than that (13.1 mg/g) of GBR. Insoluble dietary fiber content in CGBR (8.1%) was also higher than that (6.9%) of GBR, but soluble dietary fiber contents of CGBR and GBR were not differed. Lipase inhibitory and DPPH free radical scavenging activities of CGBR and GBR was also determined. CGBR showed higher lipase inhibitory activity than GBR, indicated that the increase of total phenolic compounds and phytic acid in CGBR may be involved in lipase inhibitory activity. However, DPPH free radical scavenging activity of CGBR was similar to that of GBR. We have investigated anti-obesity effect of CGBR feeding in Sprague-Dawley rat fed high fat diet. The investigation was performed for four weeks using male Sprague-Dawley rats divided into five groups: normal group (normal diet, n=8), control group (high fat diet, n=8), brown rice (BR, brown rice+high fat diet, n=8), GBR (germinated brown rice+high fat diet, n=8), and CGBR (chitosan treated germinated brown rice+high fat diet, n=8). Body weight gain and food efficiency ratio in CGBR group were significantly lower than those of control group (p
Elicitors are defined as substances that induce defence responses in plants, which include enhanced synthesis of secondary metabolites such as phenolic compounds and cell wall thickening. This study was to investigate the changes of physiologically active components in germinated brown rice treated with chitosan (CGBR) and its anti-obesity effect in Sprague-Dawley rat. Samples were prepared from the germinated brown rice treated with 0.03% chitosan containing 0.05% solar salts (CGBR) and with distilled water (GBR). Contents of physiologically active compounds such as total phenolic compounds, dietary fiber, ϒ-aminobutyric acid (GABA), and phytic acid in CGBR were significantly higher than those of GBR. Total phenolic compounds (92.1 mg/100g) in CGBR were higher than that (74.5 mg/100g) of GBR. GABA content (1882.2 nmol/g) in CGBR was about 1.5 fold higher than that (1260.0 nmol/g) of GBR. Phytic acid content (23.9 mg/g) of CGBR was higher than that (13.1 mg/g) of GBR. Insoluble dietary fiber content in CGBR (8.1%) was also higher than that (6.9%) of GBR, but soluble dietary fiber contents of CGBR and GBR were not differed. Lipase inhibitory and DPPH free radical scavenging activities of CGBR and GBR was also determined. CGBR showed higher lipase inhibitory activity than GBR, indicated that the increase of total phenolic compounds and phytic acid in CGBR may be involved in lipase inhibitory activity. However, DPPH free radical scavenging activity of CGBR was similar to that of GBR. We have investigated anti-obesity effect of CGBR feeding in Sprague-Dawley rat fed high fat diet. The investigation was performed for four weeks using male Sprague-Dawley rats divided into five groups: normal group (normal diet, n=8), control group (high fat diet, n=8), brown rice (BR, brown rice+high fat diet, n=8), GBR (germinated brown rice+high fat diet, n=8), and CGBR (chitosan treated germinated brown rice+high fat diet, n=8). Body weight gain and food efficiency ratio in CGBR group were significantly lower than those of control group (p