부산대학교 도서관

Microorganisms determine the overall biofilter performance under specific operating conditions. The toluene removal and process robustness of a laboratoryscale,ceramisite-based biotrickling filter inoculated with Burkholderia sp. strain T3 (BTFb) were compared with those of another biotrickling filter inoculated with activated sludge (BTFa) for 3 months under various operating conditions. Denaturing gradient gel electrophoresis was applied to visualise the bacterial community of the BTFa and BTFb. Real-time polymerase chain reaction was performed to determine the genes coding for toluenedegrading enzymes. Burkholderia sp. strain T3, which possesses the major toluene-degrading genes in BTFb, was traced in the BTFb bacterial community. The strain was found to stabilize the relative quantity steadily at higher than 60% during toluene biofiltration. Thus, BTFb performed more efficiently than BTFa as evidenced by achieving 98.86% toluene removal efficiency (RE) on 3 day, critical elimination capacity (EC) of 234.23 ± 10.54 g/m3/h, and rapid restoration of the initial RE and EC levels within 3 day of reoperation, even after 1 month of shutdown. The efficiency of BTFb is also evident by the stabilised RE and EC levels within a wide temperature range and a gradually decreasing system pH. Maintaining the pressure drop levels below 150 Pa during prolonged operation also contributed to the efficiency of BTFb. Thus, based on the study results,we propose that Burkholderia sp. strain T3 is a highly efficient and applicable inoculum for toluene biofiltration.