학술논문
토마토 유전자원의 점무늬병(Stemphylium lycopersici) 저항성 평가
Evaluation of Tomato Germplasm for Resistance to Gray Leaf Spot (Stemphylium lycopersici) by a Bioassay and Genotypic Analysis for the Resistance Gene (Sm)
Evaluation of Tomato Germplasm for Resistance to Gray Leaf Spot (Stemphylium lycopersici) by a Bioassay and Genotypic Analysis for the Resistance Gene (Sm)
Document Type
Article
Source
한국국제농업개발학회지, 35(4), pp.330-337 Dec, 2023
Subject
Language
한국어
ISSN
2287-8165
1225-8504
1225-8504
Abstract
본 연구는 국립농업과학원 농업유전자원센터에서 보존하고있는 223개의 토마토 유전자원을 대상으로 국내에서 분리된점무늬병 균주에 대한 저항성 자원을 선발하기 위해, 생물검정과 점무늬병 저항성 유전자(Sm)의 유전형 분석을 수행하였다. Stemphylium lycopersici 접종 2주 후 토마토 잎에서 발병도(0-4)를 조사한 결과, 발병도가 0 이상 1.0 미만인 22자원이저항성으로 판정되었고, 발병도가 1.0 이상 2.0 미만인 65자원은 중도저항성으로 판정되었다. 저항성으로 판정된 22자원은 Hybridization Probe Melting(HPM) 분석을 통해 점무늬병 저항성 유전자(Sm)의 유전형을 확인한 결과, 22자원 중 20자원이 동형접합형 저항성이었다. 본 연구에서 선발된 자원은 향후 토마토의 점무늬병 저항성품종 육성과 유전형 분석 연구 소재로 활용될 수 있을 것으로기대된다.
Gray leaf spot caused by Stemphylium spp., is a major disease of tomatoes, and it threatens its cultivation worldwide, especially in warm and humid areas. This study was conducted on 223 tomato germplasm conserved at the National Agrobiodiversity Center to select the resources resistant to the gray leaf spot pathogen strain previously isolated in Korea, using a bioassay and genotypic analysis of the resistance gene (Sm). Two weeks after inoculation with Stemphylium lycopersici, the disease index (rated on a scale of 0-4) of gray leaf spot was assessed in detached tomato leaves. The results showed that 22 resources were resistant, with a disease index of 0-1. Additionally, 65 genetic resources were found to be moderately resistant, with a disease index between 1.0 and 2.0. Subsequently, Hybridization Probe Melting (HPM) analysis of the 22 resistant genetic resources confirmed the genotype of the gray leaf spot resistance gene (Sm). Among them, 20 genetic resources showed a homozygous resistant genotype. The resources selected in this research may contribute to the breeding of new tomato varieties resistant to gray leaf spot and may serve as a basis for further genotypic analysis studies.
Gray leaf spot caused by Stemphylium spp., is a major disease of tomatoes, and it threatens its cultivation worldwide, especially in warm and humid areas. This study was conducted on 223 tomato germplasm conserved at the National Agrobiodiversity Center to select the resources resistant to the gray leaf spot pathogen strain previously isolated in Korea, using a bioassay and genotypic analysis of the resistance gene (Sm). Two weeks after inoculation with Stemphylium lycopersici, the disease index (rated on a scale of 0-4) of gray leaf spot was assessed in detached tomato leaves. The results showed that 22 resources were resistant, with a disease index of 0-1. Additionally, 65 genetic resources were found to be moderately resistant, with a disease index between 1.0 and 2.0. Subsequently, Hybridization Probe Melting (HPM) analysis of the 22 resistant genetic resources confirmed the genotype of the gray leaf spot resistance gene (Sm). Among them, 20 genetic resources showed a homozygous resistant genotype. The resources selected in this research may contribute to the breeding of new tomato varieties resistant to gray leaf spot and may serve as a basis for further genotypic analysis studies.