학술논문
Functional Epithelial Cell Line Cloned from Rat Parathyroid Glands
Document Type
research-article
Author
Source
Proceedings of the National Academy of Sciences of the United States of America, 1987 May . 84(10), 3269-3273.
Subject
Language
English
ISSN
00278424
Abstract
Primary cultures of rat parathyroid cells were developed in medium containing 5% calf serum, 1% Nutridoma-SP (a serum-free medium supplement from Boehringer Mannheim), and 0.7 mM calcium. The PT-r strain was purified by successive colony isolations and maintained differentiated characteristics (secretion of bioactive and radioimmunoactive parathyroid hormone into the culture medium, sensitivity to calcium regulation, and modulation by secretin) for 7 months in continuous culture. These cultured cells are epithelioid, display diploid chromosome numbers, and do not show a transformed phenotype. There has been no decrease in the rate of cell division or decline in parathyroid hormone secretion since the cell line was established. This clonal cell line provides an important system for further studies on the biology of the parathyroid cell.