학술논문
Effect of PERLA[R], a new cold-storage solution, on oxidative stress injury and early graft function in rat kidney transplantation model
Document Type
Report
Source
BMC Nephrology. February 22, 2024, Vol. 25 Issue 1
Subject
Language
English
ISSN
1471-2369
Abstract
Author(s): Mohamed Bejaoui[sup.1] , Chérifa Slim[sup.1] , Carmen Peralta[sup.2] and Hassen Ben Abdennebi[sup.1] Background Ischemia reperfusion injuries (IRI) after transplantation remain an unresolved issue that exposes kidney grafts to short- [...]
Background The composition of organ preservation solutions is crucial for maintaining graft integrity and early graft function after transplantation. The aim of this study is to compare new organ preservation solution PERLA[R] with the gold standard preservation solution University of Wisconsin (UW) regarding oxidative stress and early graft injury. Methods In order to assess oxidative stress after cold storage, kidney grafts have been preserved for 18 h at 4[degrees] C in either UW solution or PERLA[R] solution and then assessed for oxidative stress injury (protocol 1). To assess kidney injuries and oxidative stress after reperfusion, rat kidneys were harvested, stored in cold UW or in PERLA[R] solutions for 18 h at 4 [degrees]C and then transplanted heterotopically for 6 h (protocol 2). PERLA[R] is a high Na.sup.+/low K.sup.+ solution including PEG-35 (1 g/L), trimetazidine (1 [micro]M), carvedilol (10 [micro]M) and tacrolimus (5 [micro]M). Results Our results showed that preservation of kidneys in PERLA[R] solution significantly attenuates oxidative stress parameters after cold storage and reperfusion. We found a significant decrease in oxidative damage indicators (MDA, CD and CP) and a significant increase in antioxidant indicators (GPx, GSH, CAT, SOD and PSH). Moreover, PERLA[R] solution decreased kidney injury after reperfusion (creatinine, LDH and uric acid). Conclusion PERLA[R] solution was more effective than UW storage solution in preserving rat's kidney grafts. Keywords: Oxidative stress, Preservation solutions, Kidney transplantation, Ischemia reperfusion injury
Background The composition of organ preservation solutions is crucial for maintaining graft integrity and early graft function after transplantation. The aim of this study is to compare new organ preservation solution PERLA[R] with the gold standard preservation solution University of Wisconsin (UW) regarding oxidative stress and early graft injury. Methods In order to assess oxidative stress after cold storage, kidney grafts have been preserved for 18 h at 4[degrees] C in either UW solution or PERLA[R] solution and then assessed for oxidative stress injury (protocol 1). To assess kidney injuries and oxidative stress after reperfusion, rat kidneys were harvested, stored in cold UW or in PERLA[R] solutions for 18 h at 4 [degrees]C and then transplanted heterotopically for 6 h (protocol 2). PERLA[R] is a high Na.sup.+/low K.sup.+ solution including PEG-35 (1 g/L), trimetazidine (1 [micro]M), carvedilol (10 [micro]M) and tacrolimus (5 [micro]M). Results Our results showed that preservation of kidneys in PERLA[R] solution significantly attenuates oxidative stress parameters after cold storage and reperfusion. We found a significant decrease in oxidative damage indicators (MDA, CD and CP) and a significant increase in antioxidant indicators (GPx, GSH, CAT, SOD and PSH). Moreover, PERLA[R] solution decreased kidney injury after reperfusion (creatinine, LDH and uric acid). Conclusion PERLA[R] solution was more effective than UW storage solution in preserving rat's kidney grafts. Keywords: Oxidative stress, Preservation solutions, Kidney transplantation, Ischemia reperfusion injury