학술논문
A single-nucleotide substitution of CjTKPR1 determines pollen production in the gymnosperm plant Cryptomeria japonica
Research Report
Cryptomeria japonica
Research Report
Cryptomeria japonica
Document Type
Report
Author
Kakui, Hiroyuki; Ujino-Ihara, Tokuko; Hasegawa, Yoichi; Tsurisaki, Eriko; Futamura, Norihiro; Iwai, Junji; Higuchi, Yuumi; Fujino, Takeshi; Suzuki, Yutaka; Kasahara, Masahiro; Yamaguchi, Katsushi; Shigenobu, Shuji; Otani, Masahiro; Nakano, Masaru; Nameta, Masaaki; Shibata, Shinsuke; Ueno, Saneyoshi; Moriguchi, Yoshinari
Source
PNAS Nexus. August 2023, Vol. 2 Issue 8
Subject
Language
English
ISSN
2752-6542
Abstract
Introduction Pollen production is critical for plant reproduction; therefore, the genetics of pollen development have been studied extensively. This research has led to the discovery of the pollen developmental pathway, [...]
Pollinosis, also known as pollen allergy or hay fever, is a global problem caused by pollen produced by various plant species. The wind-pollinated Japanese cedar (Cryptomeria japonica) is the largest contributor to severe pollinosis in Japan, where increasing proportions of people have been affected in recent decades. The MALE STERILITY 4 (MS4) locus of Japanese cedar controls pollen production, and its homozygous mutants (ms4/ms4) show abnormal pollen development after the tetrad stage and produce no mature pollen. In this study, we narrowed down the MS4 locus by fine mapping in Japanese cedar and found TETRAKETIDE [alpha]-PYRONE REDUCTASE 1 (TKPR1) gene in this region. Transformation experiments using Arabidopsis thaliana showed that single-nucleotide substitution ("T" to "C" at 244-nt position) of CjTKPR1 determines pollen production. Broad conservation of TKPR1 beyond plant division could lead to the creation of pollen-free plants not only for Japanese cedar but also for broader plant species. Keywords: pollinosis, gymnosperm, conifer, pollen, male-sterility, Japanese cedar
Pollinosis, also known as pollen allergy or hay fever, is a global problem caused by pollen produced by various plant species. The wind-pollinated Japanese cedar (Cryptomeria japonica) is the largest contributor to severe pollinosis in Japan, where increasing proportions of people have been affected in recent decades. The MALE STERILITY 4 (MS4) locus of Japanese cedar controls pollen production, and its homozygous mutants (ms4/ms4) show abnormal pollen development after the tetrad stage and produce no mature pollen. In this study, we narrowed down the MS4 locus by fine mapping in Japanese cedar and found TETRAKETIDE [alpha]-PYRONE REDUCTASE 1 (TKPR1) gene in this region. Transformation experiments using Arabidopsis thaliana showed that single-nucleotide substitution ("T" to "C" at 244-nt position) of CjTKPR1 determines pollen production. Broad conservation of TKPR1 beyond plant division could lead to the creation of pollen-free plants not only for Japanese cedar but also for broader plant species. Keywords: pollinosis, gymnosperm, conifer, pollen, male-sterility, Japanese cedar