부산대학교 도서관

Background This study aimed to investigate the intestinal carrier status of Enterococcus spp. among children in a pediatric intensive care unit (PICU) and reveal the role of hospitalization in the alteration of resistance phenotypes and clonal diversity of the isolates during admission and discharge periods. Methods Two separate stool samples were collected from hospitalized patients in the pediatric intensive care unit at admission and discharge times. The culture was done, and Enterococcus species were tested for antimicrobial susceptibility and carriage of vanA-D gene subtypes. Random Amplified Polymorphic DNA (RAPD)-PCR was used for a phylogenetic study to check the homology of pairs of isolates. Results The results showed carriage of Enterococci at admission, discharge, and at both time points in 31%, 28.7%, and 40.1% of the cases, respectively. High frequencies of the fecal Enterococcus isolates with vancomycin-resistance (VR, 32.6% and 41.9%), high-level of gentamicin-resistance (HLGR, 25.6% and 27.9%), and multi-drug resistance phenotypes (MDR, 48.8% and 65.1%) were detected at admission and discharge times, respectively. Resistance to vancomycin, ampicillin, and rifampicin was higher among E. faecium, but resistance to ciprofloxacin was higher in E. faecalis isolates. The increased length of hospital stay was correlated with the carriage of resistant strains to vancomycin, ampicillin, and ciprofloxacin. While the homology of the isolates was low among different patients during hospitalization, identical (9%) and similar (21%) RAPD-PCR patterns were detected between pairs of isolates from each patient. Conclusions The high rate of intestinal carriage of VR, HLGR-, and MDR-Enterococci at admission and during hospitalization in the PICU, and the impact of increased length of hospital stay on the fecal carriage of the resistant strains show the importance of antibiotic stewardship programs to control their transmission and spread in children. Keywords: Enterococcus, Pediatric Intensive Care Unit, Carriers, MDR, VRE, HLGR, Antimicrobial Resistance
Author(s): Fariba Shirvani[sup.1], Romina Hassanzadeh[sup.2], Bahareh Attaran[sup.2], Ghazale Ghandchi[sup.1], Nafiseh Abdollahi[sup.1], Zari Gholinejad[sup.1], Zahra Sheikhi[sup.3], Azita Behzad[sup.4], Fatemeh Fallah[sup.1], Leila Azimi[sup.1], Azam Safarkhani[sup.3], Abdollah Karimi[sup.1], Alireza Mahdavi[sup.5], Shahnaz Armin[sup.1], Roxana [...]