학술논문
The natural cytotoxic receptors (NKp30, NKp44 and NKp46) gene expression in Coeliac disease patients
ORIGINAL ARTICLE
ORIGINAL ARTICLE
Document Type
Academic Journal
Author
Source
New Zealand Journal of Medical Laboratory Science. July 2023, Vol. 77 Issue 2, p66, 5 p.
Subject
Language
English
ISSN
1171-0195
Abstract
INTRODUCTION Coeliac disease (CD) is a chronic gastrointestinal autoimmune disorder that occurs in genetically predisposed individuals due to abnormal immune responses to gluten proteins (1, 2). The disease can present [...]
Objectives: Defects in the expression of natural killer (NK) cells activating receptors (like NKp30, NKp44, and NKp46) impair the ability of NK cells to kill virus-infected cells and are associated with amplified local inflammation. This study aimed to evaluate the expression of these receptors in coeliac disease (CD) patients relative to controls. Methods: We analysed the NKp30, NKp46 and NKp44 genes expression in intestinal biopsy specimens of 40 confirmed coeliac disease patients and 40 controls using the real-time PCR method. Results: The NKp30 mRNA expression in coeliac disease patients was significantly lower than in controls (p=0.01) with a significan inverse correlation with the patient's weight loss symptom (p=0.0173). The NKp44 mRNA expression was extremely higher in coeliac disease patients than in controls (p=0.02). The mRNA expression of NKp46 did not differ between patients and controls (p=0.3). The expression levels of these receptors did not show any significant difference between Marsh II and Marsh III groups of patients and controls (p>0.05). Conclusion: Further studies are needed to elucidate the predominant NKp30 isoform and possible SNP role in controlling its expression in coeliac disease which leads to designing innovative targeted therapy for coeliac disease patients. Keywords: Autoimmune Diseases, Biopsy, Coeliac disease (CD), Gene Expression
Objectives: Defects in the expression of natural killer (NK) cells activating receptors (like NKp30, NKp44, and NKp46) impair the ability of NK cells to kill virus-infected cells and are associated with amplified local inflammation. This study aimed to evaluate the expression of these receptors in coeliac disease (CD) patients relative to controls. Methods: We analysed the NKp30, NKp46 and NKp44 genes expression in intestinal biopsy specimens of 40 confirmed coeliac disease patients and 40 controls using the real-time PCR method. Results: The NKp30 mRNA expression in coeliac disease patients was significantly lower than in controls (p=0.01) with a significan inverse correlation with the patient's weight loss symptom (p=0.0173). The NKp44 mRNA expression was extremely higher in coeliac disease patients than in controls (p=0.02). The mRNA expression of NKp46 did not differ between patients and controls (p=0.3). The expression levels of these receptors did not show any significant difference between Marsh II and Marsh III groups of patients and controls (p>0.05). Conclusion: Further studies are needed to elucidate the predominant NKp30 isoform and possible SNP role in controlling its expression in coeliac disease which leads to designing innovative targeted therapy for coeliac disease patients. Keywords: Autoimmune Diseases, Biopsy, Coeliac disease (CD), Gene Expression